FEBS Practical Course 2016: "Microspectroscopy: Functional Imaging of Biological Systems"


Every two years, the MicroSpectroscopy Centre Wageningen of Wageningen University and Microscopic Imaging Centre of Radboud Institute for Molecular Life Sciences organise a microspectroscopy course for young scientists and PhD students. This year we organise this course in collaboration with Dr. Y.Stahl and Dr. S. Weidtkamp-Peters from Centre for Advanced Imaging of Heinrich Heine University in Germany.

During this course, you will learn several microscopic and spectroscopic techniques for studying biological processes in living cells. Microspectroscopic applications are the methods of choice because direct information on molecular interactions and dynamic events involving biomolecules is obtained with minimal perturbation of cellular integrity and function.

The course will cover the following microscopic and spectroscopic techniques:


Confocal microscopy


Multiphoton microscopy


Photo-activatable imaging


Single particle tracking


Total internal reflection fluorescence microscopy


Super-Resolution and Correlative Microscopy


Förster resonance energy transfer (FRET)


FRET imaging techniques


Fluorescence lifetime imaging (FLIM)


Fluorescence Correlation Spectroscopy (FCS)


Multi dimensional Fluorescence


Fluorescence recovery after Photobleaching (FRAP)




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Our participants had a great time during the course!

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